diff --git a/.github/ISSUE_TEMPLATE/announcement.yml b/.github/ISSUE_TEMPLATE/announcement.yml
index 3c414cbc..ba4b53b8 100644
--- a/.github/ISSUE_TEMPLATE/announcement.yml
+++ b/.github/ISSUE_TEMPLATE/announcement.yml
@@ -30,7 +30,6 @@ body:
     attributes:
       label: Virtual environments affected
       options:
-        - label: Ubuntu 16.04
         - label: Ubuntu 18.04
         - label: Ubuntu 20.04
         - label: macOS 10.15
diff --git a/.github/ISSUE_TEMPLATE/bug-report.yml b/.github/ISSUE_TEMPLATE/bug-report.yml
index 61b05c56..fea945a8 100644
--- a/.github/ISSUE_TEMPLATE/bug-report.yml
+++ b/.github/ISSUE_TEMPLATE/bug-report.yml
@@ -12,7 +12,6 @@ body:
     attributes:
       label: Virtual environments affected
       options:
-        - label: Ubuntu 16.04
         - label: Ubuntu 18.04
         - label: Ubuntu 20.04
         - label: macOS 10.15
diff --git a/.github/ISSUE_TEMPLATE/tool-request.yml b/.github/ISSUE_TEMPLATE/tool-request.yml
index 87cee793..9c2a86d5 100644
--- a/.github/ISSUE_TEMPLATE/tool-request.yml
+++ b/.github/ISSUE_TEMPLATE/tool-request.yml
@@ -51,7 +51,6 @@ body:
     attributes:
       label: Virtual environments affected
       options:
-        - label: Ubuntu 16.04
         - label: Ubuntu 18.04
         - label: Ubuntu 20.04
         - label: macOS 10.15
diff --git a/README.md b/README.md
index 491922e0..fe6239d5 100644
--- a/README.md
+++ b/README.md
@@ -48,7 +48,6 @@ You can also track upcoming changes using the [awaiting-deployment](https://gith
 
 [ubuntu-20.04]: https://github.com/actions/virtual-environments/blob/main/images/linux/Ubuntu2004-README.md
 [ubuntu-18.04]: https://github.com/actions/virtual-environments/blob/main/images/linux/Ubuntu1804-README.md
-[ubuntu-16.04]: https://github.com/actions/virtual-environments/blob/main/images/linux/Ubuntu1604-README.md
 [windows-2022]: https://github.com/actions/virtual-environments/blob/main/images/win/Windows2022-Readme.md
 [windows-2019]: https://github.com/actions/virtual-environments/blob/main/images/win/Windows2019-Readme.md
 [windows-2016]: https://github.com/actions/virtual-environments/blob/main/images/win/Windows2016-Readme.md